Jci acquired cisplatin resistance in human ovarian cancer. The aim of this study was to determine whether cisplatinresistant cells derived from hela cells have an apoptosisresistant phenotype. There are some common chemotherapy drugs, such as cisplatin, docetaxel. Anderson cancer center, 1515 holcombe boulevard, houston, tx 770304009, usa cisplatin is one of the most potent antitumor agents. For the lung is the firstline to contact with the atmosphere, in which the oxygen content is 21%, so 21% o2 is commonly used as.
Hypoxiainduced autophagy mediates cisplatin resistance in. Cisplatina resistant hela cells are resistant to apoptosis. Sphase arrest on hela cells, cisplatin induced g1 arrest, while combination of ceo and cisplatin induced g2m arrest. Tunable biodegradable nanocomposite hydrogel for improved cisplatin efficacy on hct116 colorectal cancer cells and decreased toxicity in.
Potential implications of grp58 expression and susceptibility. Hela cell proliferation the results from mtt assay showed a concentration and time dependent reduction in the proliferation rate of hela cells on treatment with cidanandcisplatin figure 1. In addition, cisplatin induces apoptosis with the caspasedependent pathway. The development of anticancer rutheniumii rsc publishing. Less than 30% of malignant gliomas respond to adjuvant chemotherapy. A cisplatinresistant cell subline, hela cddp cells, showed a 19fold resistance to cisplatin compared with the parent cells. Platinum accumulation in both sensitive and resistant cell lines. The effect of combined treatment of hela cells with cisplatin. The half maximal inhibitory concentration ic 50 of cisplatin in hela and siha cells at 72 hours was. Doseresponse curves for hela, siha, vero, and 3t3 cell. We sought to investigate if gleevec has a potential role in treatment of nonsmall cell. Gleevec aka sti571, imatinib is a recently fda approved antitumor drug for chronic myelogenous leukemia. A cisplatin resistant cell subline, hela cddp cells, showed a 19fold resistance to cisplatin compared with the parent cells. However, the resistant cell line was twofold more efficient at effluxing drug, which was associated with reduced total drug accumulation for equivalent.
Induction of cisplatin resistance in a2780 cell line cisplatin resistant a2780 cell lines were derived from original parental cell line by continuous exposure to stepwise increasing concentrations of cisplatin. P ic 50 of cisplatin for each cell line in rpmi 1640 containing 10% fbs and 1% penicillinstreptomycin solution. Cytotoxicity cisplatinresistant tumor lines ic 50 m cancer cell line cisplatin 1 chronic myelogenous leukemia k562 80 3 chronic myelogenous leukemia kg1 48 2 acute myelogenous leukemia ml2 80 1 mouse melanoma b16 80 6 colon cancer ht29n 80 12 colon cancer ht29 50 8 colon cancer hct116 80 9 lung carcinoma a427 63 6 breast carcinoma. Preparation and cytotoxicity of cisplatincontaining liposomes. Cisplatin has a number of side effects that can limit its use. M, respectively p cisplatin is particularly effective against testicular cancer. An equitoxic dose ic50 of cisplatin produced dna fragmentation in hela cells but not in hela cddp cells. Cinnamon essential oil, cisplatin, hela cells, apoptosis, cell. Apexbio cisplatininhibits dna synthesis,chemotherapy drug. Jan 17, 2008 two models were developed from h69 sclc cells, h69. Table s1 ic50 values for a0 and cisplatin in human cancer cells ic50. Meanwhile, the ic50 of thymoquinone in hela and siha cells was 29. Sensitivity to cisplatin in primary cell lines derived from.
The treatment of the cells with cisplatin or bromelain alone resulted in a dosedependent and a timedependent decrease in the viability of the mdamb231 cells at 24 and 48 h, with higher concentrations and longer treatment times resulting in lower levels of cell viability fig. Ratio of cisplatin ic50 to compound ic50 for mouse b16f10. When hela cells were irradiated in the presence of cisplatin under hypoxic conditions, the resulting c e d lo. Systematic identification of genomic markers of drug. Ci value of administration of metformin and cisplatin in hela cells. Following the 48 and 72h hour treatments at 37c, cells were. The inhibitory effect of cisplatin on hela cell growth was analyzed by the mtt assay. Combination of ceo and cisplatin decreased cells viability rather than cisplatin solely fig. In cochlear cells, cisplatin treatment results in the increase of caspases3 and 9 and causes the cochlear damage side effect 1. M, cisplatin sensitivity increased in both cell lines compared to cisplatin treatment alone.
M, respectively p cisplatin in cervical cancer cell line hela and its mechanism, cell growth inhibition of cisplatin on hela cells was analyzed by mtt assay. Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant. Mar 28, 2012 cells from ewings sarcoma were more sensitive to olaparib p 2. The half maximal inhibitory concentration ic50 of cisplatin in hela and siha cells at 72 hours was. Cisplatin doseresponse curve of hela cells treated in suspension with 4 pgml cisplatin for one to 4 h under aerobic a or hypoxic a conditions. Jul 23, 2015 hypoxia enhanced the cisplatin resistance of lung cancer cells. The effects of cisplatin on transcription of e6 were analyzed by rtpcr. The effect of cisplatin on transcription of hpv e6 was analyzed by rtpcr and protein expression. Inhibition of the cervical cancer growth by cidan and cisplatin. Cell death in hela cells upon imperatorin and cisplatin treatment. Increase in the concentration of cidan from 10 to 30 mg mlled to decrease in the proliferation rate of hela cells from 95. The cytotoxicity of email protected coreshell nanocrystals, evaluated by mtt assay, shows a much lower ic50 35.
Gleevec binds specifically to bcrabl tyrosine kinase and inhibit the tyrosine kinase activity. Generation of cisplatinresistant ovarian cancer cell lines. Every two months, cell survival was analyzed by mtt assay. Synthesis and cytotoxicity evaluation of novel asymmetrical. Cell apoptosis was examined by cytometry and hoechst33258 staining after treatment with cisplatin. The cytotoxicity was evaluated by sulforhodamine b assay against a549, b16f10, hct15, skmel2 and skov3 cell lines and compared with standard drug cisplatin. Inhibitory concentration ic50 of cisplatin and metformin was determined before. Ratio of cisplatin ic50 to compound ic50 for human a431 cells. Combination of cisplatin and cinnamon essential oil. This complex induced death in hela cells, with an ic50. Then, cell viability was assessed using mtt assay and ic50 was determined.
After transfection, the ic50 value of siha and hela cells was examined by cck 8. Some experiments have found that cisplatin can inhibit protein expression in hela cells of cervical cancer cell lines, promote cell apoptosis significantly and inhibit the growth to achieve a. Combination of cisplatin and bromelain exerts synergistic. Percentage inhibition of a549 lung cancer cells by cisplatin. Pdf metformin enhances antiproliferative effect of cisplatin in. M cell line a 0 cisplatin shsy5y 50 2 pnt1 10 5 sw872 50 5 rd 21 6 a375 42 6 hep2 28 7 mcf7dx 50 7 ht1080 12 9 hos 26 10 saos2 50 11 pc3 34 12 hepg2 48 12 hela 16 mcf7 50 15 panc1 50 15 caco2 17 a431 22 20 u2os 34 20 ht29 50 22. Apoptosis is the main response of cells to chemotherapeutic agents including cisplatin. Cells were then treated with 100 l compound of concentrations between 6. Cp and h69cis200 using 100 ngml or 200 ngml cisplatin respectively. Dipyridamole increases the cytotoxicity of cisplatin in human. The cell growth inhibition of cisplatin on hela cells was analyzed by mtt assay. The subline showed a collateral sensitivity to paclitaxel.
Doseresponse curves for hela, siha, vero, and 3t3 cell openi. Combination of cisplatin and cinnamon essential oil inhibits. Understanding cisplatin resistance using cellular models. Cisplatin activates sapkjnk, p38 kinase, and erk dose dependently in cisplatin sensitive hela cells, whereas cisplatin mediated activation of sapkjnk is significantly reduced in the cpr subline. Selection of our books indexed in the book citation index in web of. In the presence of 30 m dip, the ic 50 of cisplatin was reduced by 39% for both cell lines. Vogels textbook of practical organic chemistry, 5th edition. Cell death in hela cells upon imperatorin and cisplatin treatment article pdf available in folia histochemica et cytobiologica 503. Here, we have asked whether variations in the constitutive expression of earlygrowth response factor 1 egr1 predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics.
To quantitate the sensitisation of cells to chemotherapeutics after infection with aav2, doseresponse curves were established. The mechanism of cisplatininduced apoptosis in hela cells. Cisplatin sensitivity is enhanced in nonsmall cell lung. Compound 5f outperformed cisplatin against a549, hct15, skmel2 and b16f10 cell lines.
Cisplatin and doxorubicin were added twice a week after reseeding. Mir1284 enhances sensitivity of cervical cancer cells to cisplatin. Enhanced sensitivity of small cell lung cancer cell lines to. Meanwhile, the ic 50 of thymoquinone in hela and siha cells was 29. Full text molecular mechanisms of cisplatin resistance in. The a2780cp70 cell line was fold more resistant to cisplatin than a2780 cells. In this study, t47d cells were treated with various concentrations of cisplatin 0, 3. Combination assay was done by combined ceo and cisplatin on hela cells with serial concentrations of 120, 18, 14, and 12 ic 50 of ceo and cisplatin. Pdf the changing 50% inhibitory concentration ic50 of cisplatin. Issues surrounding standard cytotoxicity testing for assesing. With a novel cell biosensor chip system allowing continuous monitoring of respiration, glycolysis, and impedance we followed cisplatin treatment of different cancer cell lines in real. Combination index ci was calculated to evaluate the.
We determined the relative proliferation of the cell lines after mockinfection pbs or aav2 infection with 10 3 or 10 4 tcidcell and subsequent treatment with various concentrations of cisplatin or etoposide or a combination of both drugs. Cisplatin induces down regulation of bcl2 in t47d breast. Since the discovery of cisplatin more than 40 years ago and its clinical introduction in the 1970s an enormous amount of research has gone into elucidating the mechanism of action of cisplatin on tumor cells. Cell viability was evaluated with the resazurin assay see materials and methods. Cinnamon essential oil, cisplatin, hela cells, apoptosis, cell cycle. The study of cytotoxicity to glc4 and glc4 cddp cell lines showed similar ic50 values approximately 1. The genomics of drug sensitivity in cancer project is a collaboration between the cancer genome project at the wellcome sanger institute uk and the center for molecular therapeutics, massachusetts general hospital cancer center usa. Cell apoptosis was measured with flow cytometry and hoechst 33258 staining following cisplatin treatment. Cisplatin was also found to cause ros generation and increase lipid peroxidation, which leads cells to the apoptotic pathway. When studied at their respective ic50 doses, drug accumulation rates were similar for the two cell lines. A2780s cells were more susceptible to both cisplatin and carfilzomib while analyses of drug interactions between the two agents showed synergistic effects in all affected fractions of drugtreated a2780s and a2780cp cells ci0. Original article inhibition of the cervical cancer growth.
The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosispromoting effects on hela cells p cisplatin in vitro in hela cells cervical cancer cell line. Relationship between ic 50 values and cell seeding densities. It crossreacts with another two important membrane tyrosine kinase receptors, ckit and pdgf receptors. After the indicated incubation times in cisplatin media the cells were harvested with trypsinization and incubated in a propidium iodide cocktail 50 gml propidium iodide sigma, 5 gml rnase a, 0. Covid19 is an emerging, rapidly evolving situation. Cidan and cisplatin reduced the viability of hela cells at 48 h to 43. The concentrations able to inhibit 50% viability ic50 were calculated and cell lines were ordered according to the ic50 value for cisplatin. Phytochemical content an in vitro toxicity of glycine.
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